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5-day BOD and COD can be removed by biological aerobic treatment of saline wastewater. In this research, halophilic microorganisms, namelyAspergillus oryzaeandHalobacillus dabanensiswere isolated from a return sludge basin of a wastewater treatment plant in the City of Bandar Abbas in southern Iran , that contained a Total Dissolved Solid (TDS) of about 7500 mg l^-1. These microorganisms (bacteria and fungi) could tolerate 20% concentration of salt (NaCl) in Sabouraud-4% dextrose agar and Sabouraud-2% dextrose broth medium and brain heart (BHI) agar and BHI broth medium. The films ofAspergillus oryzaeandHalobacillus dabanensiswere formed around the Ca-alginate. These bioflims were introduced to a fixed bed column, on top of which saline wastewater was released with flow rates of 2-6 ml min^-1. According to the results of Stover-Kincannon model, the constant values of maximum BOD and COD were estimated at 0.066 mg BOD₅l^-1min^-1and 0.1449 mg COD l^-1min^-1, respectively. The saturation constant values, at the flow rate of 2ml min^-1, byAspegillus oryzaewere 0.00003 mg BOD₅l^-1min^-1and 0.00038 mg COD l^-1min^-1. The removal process in fixed bed column was stopped after 1200 minutes.