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Introduction

Industries are responsible for the release of billion gallons of wastewater containing high levels of salt and organic matters¹into the environment. Such a huge amount of waste generated in the world is capable of being reused as a source of water supply in areas with water shortages.²More efficient treatment methods should be utilized before reusing and/or discharging the saline wastewater to the environment.³

Table Click here to View table

Salinity is known to have toxic influences on bacteria and is also capable of altering the microbial community.⁴It can affect the metabolism of microorganisms.⁵Excessive salt content in wastewater inhibits large number of enzymes and causes salt stress among microbial species.^{6, 7}

This can decrease biological⁷and / or cellular activities and ultimately leads to plasmolysis.⁶It would be a rather high-priced procedure to remove salinity by physicochemical process in advance of biological treatment [8]. Regardless of being inconvenient, bio-treatment of saline wastewater is cost-effective and environment friendly.⁵盐废水的生物法我的效率s poor especially for the removal of COD. This is mainly because of negative impact of salt on microbial flora and augmentation of suspended solids in the effluent mainly at high concentration of salt (>2%).^9,10突然的盐度变化影响着bio-tr不利eatment process more than fluctuation within a limited range, which widely inhibits aerobic bio-treatment.³According to the literature, aerobic treatment is affected negatively in case chloride exceeds 5000-8000 mg l^-1.¹²Halophilic bacteria have been recommended by some researchers for bio-treatment of saline wastewater.¹³Sequencing Batch Reactor (SBR) is a satisfactory system for bio-treatment at high salinity, aerobic biosolids have poor settlement due to effluent turbidity, and membrane coupled bioreactor including Ultra Filtration (UF) or Micro-Filtration (MF).⁵

Some halophilic microbes are able to improve treatment within a wide range of salinity and are adaptable to salinity shock.¹¹Salinity tolerance is the primary way to enhance efficiency of bio-treatment of hypersaline wastewater. It should be mentioned that microbes could grow at the salinity range of 0-15% while halophilics tolerate grow well at the salinity range of 1-30%.¹¹Accordingly, halophilics survive at high salinity.¹⁴

The microbial structure of aerobic granules is denser and thus they settle rapidly, and furthermore they could bear the incoming shocks and are tolerant of medium toxic environments.¹⁵Extracellular Polymeric Substances (EPS) are a combination of polysaccharides, nucleic acids and lipids.¹⁶

Immobilization may be an impressive method to inhibit biomass from being washed out; when environmental factors are not helpful, or there are available toxic substrates are present.¹⁷The feasible influence of salt on immobilized biomass has given less notice so far due to different studies utilizing disparate operating situations and microbial communities.¹⁸

According to the report, high concentrations of salt (NaCl) can decrease membrane penetrability.⁵Reid et al. used an immersed Membrane Bioreactor (MBR) to study the impacts of salinity shocks (up to 5000 mg l^-1) on qualifications of activated sludge as well as the penetrability of membrane.¹⁹Sun et al.²⁰investigated the impacts of salinity at deferent salt concentrations of (1000 mg l^-1, 2500 mg l^-1, 5000 mg l^-1, 15,000 g l^-1NaCl) on a biofilm-MBR process for shipboard wastewater treatment. Results demonstrated that the membrane fouling rates between 0.45 mbar day^-1and 0.47 mbar day^-1for 0 mg l^-1and 1000 mg l^-1salt, respectively [20]. High salinity effects on nitrification and denitrification have already been studied.²¹然而,有氧造粒机制在treatment of saline wastewater is still unknown.⁷Bio-treatment of hypersaline wastewater by pure halophilic bacteria has been investigated in various biofilms and SBR.^14,22

In this research, a fixed bed reactor containing biofilm of fungi and bacteria species was used for removal of BOD₅and COD from saline wastewater. The fungi and bacteria species reused from a municipal wastewater treatment plant located in Bandar Abbas City, southern Iran. The preferences of an aerobic fixed bed include (i) stubby Hydraulic Retention Time (HRT), (ii) high specific surface area, (iv) in sensitivity to toxic substrate, low energy consumption, and (vii) simplicity of operation.

Materials and Methods

Optimizing growth conditions of fungi and bacteria

In this study, the returned activated sludge a municipal wastewater treatment plant in Bandar Abbas City was sampled for purification of fungi and bacteria species according to the relevant standard methods. The species of fungi and bacteria were identified by Polymerase Chain Reaction (PCR). The species were cultured in Sabouraud-4% dextrose agar (Merck) and brain heart (BHI) agar (Merck) medium with 5 to 25% NaCl concentrations to determine the tolerance limit of the fungi and bacteria to medium salinity. Temperature range for the growth of fungi and bacteria species was set between 28- 50ºC in order to obtain the optimized temperature. Fungi and bacteria species were used for the formation of biofilm around the Ca-alginate bed and treatment of the saline wastewater.

Formation of biofilm

10 g of sodium alginate powder was added to 50-60 times that of distilled water. To make it humanized, the mixture was heated by a heater. Then, the solution was poured into a 0.2 M solution of CaCl₂to form Ca-alginate granules.²³The Ca-alginate granules have to be rotating during the formation. The solution was placed in a refrigerator for 24 h to make the granules stronger. The Ca- alginate granules were poured in the sterile Sabouraud-2% dextrose broth (Merck) and BHI broth (Merck) medium; and autoclaved. Fungi and bacteria species were inoculated into Sabouraud-2% dextrose broth and BHI broth medium based on the optimized amounts of inoculation. It is worth mentioning that the optimum inoculation amounts of fungi and bacteria species were calculated based on the dry mass and Mcfarland standards,²⁴respectively. They were placed in a shaker with a rotation speed of 150 rpm at the optimized temperature. This process was studied with Scanning Electron Microscopy (SEM) image in order to make sure of the formation of fungi and bacteria films around the Ca- alginate.

Fixed bed column

固定床反应器cylindr的形式ical column; 50 cm in height and 4.5 cm in diameter. It was made of glass (Fig.1). The wastewater in the fixed bed column was taken from the Grit Chamber. Saline wastewater was filtered through filter papers of Whatman GF-Ctype to prevent blockage of the column due to entry of suspended particles and colloids. After the biofilms (fungi and bacteria) were formed around the Ca-alginate; was added to the column. It was aerated from the bottom by an aquarium pump. In order to adapt the environmental conditions, the fungi and bacteria films were settled in the column, respectively for 43 h and 24 h, to adapt to the conditions of the environment. Meanwhile, the brownish light-green pigments were formed in the fungi biofilms. Omil et al. (1995) showed it is possible to adopt an active methanogenic biomass at the salinity content as same as the effluent. They concluded that the process performance depends on appropriate strategies for adaptation of the biomass to high salinity.²⁵

Figure 1 Click here to View figure

After the period of 43 and 24 h, the municipal wastewater with concentrations of TDS 7500 mg l^-1and the flow rates of 2, 4 and 6 ml min^-1was conducted to the column from top. The amounts of COD and BOD₅were measured every 24 h.²⁶The pH of saline wastewater was measured with HORIBA model pH meter (F-12) and was found to be 8 based on batch operation. The pH was controlled every day.

Kinetic Model

The kinetics of substrate (COD and BOD₅) elimination in the bioreactor was estimated a modified Stover–Kincannon model that, at a steady state, has the following form²⁷:

ds/dt=(U_max)Q.S_i)/V)/(K_B+(Q.S_i)/V) (1)

Form (1) may be liberalized as

(ds/dt)^-1=V/Q/(S_i-S_e)=K_B/U_max.V/Q/S_i+1/U_max(2)

Form (2) may be useful to present for showing the graph plot that associates with the inversion of the substrate loading removal rate (V/Q/(Si - Se)) versus the total substrate loading rate (V/Q/Si). If the plot is linear, linear regression can be used to appraise the intercept and the slope. The outcome is a straight line portion of intercept 1/ U_maxand a slope of K_B/U_max. K_Bis suffusion constant (mg l^-1min^-1) and U_maxis a constant for the maximum substrate removal rate (mg l^-1min^-1).

Results and Discussion

Determining best growth conditions of bacteria and fungi

Based upon PCR experiments, the purified fungi and bacillus wereAspergillus oryzaeandHalobacillus dabanensis, respectively. The growth ofAspergillus oryzaeandHalobacillus dabanensiswas studied in Sabouraud-4% dextrose agar and BHI agar medium with different concentrations of NaCl of 5%, 10%, 15%, 20% and 25%. In the Sabouraud-4% dextrose agar and BHI agar medium, the best growth was obtained at NaCl concentration of 20%. For two microorganisms, the highest level of dry mass and the best growth were achieved at NaCl concentration of 20% in Sabouraud-2% dextrose broth and BHI broth medium. No more significant growth was observed in the medium at other NaCl concentrations.Aspergillus oryzaehad the best growth at the temperature of 37^ºC which was procured after 72 h of single colonies with the brownish green pigments in the Sabouraud-4% dextrose agar medium. At temperature 28^ºC and 45^ºC, no growth was observed. However,Halobacillus dabanensishad the best growth at the temperature of 45^ºC released after 24-36 h of single colony.

Biofilm of Aspergillus oryzae and Halobacillus dabanensis

The optimum inoculation ofAspergillus oryzaeinto the saline wastewater and Sabouraud-2% dextrose broth medium for formation of biofilm were 15 ml and 8 ml, respectively. Optimum inoculation ofHalobacillus dabanensisinto BHI broth medium and saline wastewater for formation of biofilm were 5 Mcfarland to the amount of 3% and 8 Mcfarland to the amount of 5%. After 56 h,Aspergillus oryzae成长在Ca-alginate床,30小时后,the biofilm ofHalobacillus dabanensiswas organized. To ensure the formation of fungi and bacillus films, the samples of Ca-alginate granules were imaged by SEM (Fig.2 and Fig.3 ). On the other hand, as theAspergillus oryzaeandHalobacillus dabanensiswere formed around Ca-alginate with optimum conditions in the saline wastewater, no significant thickness ofAspergillus oryzaeandHalobacillus dabanensiswas observed around Ca-alginate bed recorded by SEM images; therefore, it was disregarded set the Ca-alginate granules in the fixed bed column.

Figure 2: SEM image of biofilm ofAspergillus oryzaearound the Ca- alginate. Click here to View figure

Figure 3: SEM image of biofilm ofHalobacillus dabanensisaround the Ca- alginate. Click here to View figure

Effect of flow rates on removal of BOD₅and COD by biofilms of (Aspergillus oryzaeandHalobacillus dabanensis) and, determination of kinetic constants

Biofilm is a conspicuous method upgrading the performance of bioreactors in removal of environmental pollution. In this research, the performance of bioreactors was analyzed taking into account the efficiencies of BOD₅and COD removal depending on the flow rates ranging between 2 to 6 ml min^-1. According to the obtained results, when flow rate was increased from 2 to 6 ml min^-1, the amounts of volumetric loading rate increased for both kinds of pollution. Tables 1 and 2 give the effect of flow rates on efficiency of BOD₅and COD removal byAspergillus oryzaeandHalobacillus dabanensis. An increase in the flow rate from 2 to 6 ml min^-1cased a decrease in removal of BOD₅and COD. This was due to the fact that in the high flow rates, the contact times of saline wastewater with biofilms as well as removal of BOD5 and COD content of saline wastewater in the column are decreased. The removal of BOD₅byAspergillus oryzaeandHalobacillus dabanensisdecreased from 105 mg l^-1to 10 mg l^-1, and from 105 mg l^-1to 20 mg l^-1, respectively. Moreover, the removal of COD byAspergillus oryzaeandHalobacillus dabanenswas decreased from 245 mg l^-1to 34 mg l^-1and from 245 mg l^-1to 45 mg l^-1, respectively. These results show thatAspergillus oryzaeandHalobacillus dabanensiscan efficiently remove BOD₅and COD to the amounts of 90.4761% and, 86.1224%, 80.9523% and 81.6326%, respectively. Increased surface of biofilm inAspergillus oryzaecaused the maximum removal of BOD₅and COD, in comparison withHalobacillus dabanensisbiofilm. This increased the volumetric mass transfer rate. Although biological treatment of saline wastewater is possible by using holophilic microorganisms; however, such microorganisms can tolerate salt to some extent. Accordingly, in contact with low salinity medium, after absorbing a large amount of salt, their tolerance will reduce and their cytoplasm will disintegrate and vice versa.

Table 1: Effect of hydraulic retention time on COD and BOD₅loading rate and on efficiencies of COD and BOD₅removal byAspergillus oryzae.

HRT (min)	Q (ml min-1)	VLRCOD (mg COD l-1min-1)	VLRBOD5 (mg BOD5l-1min-1)	ECOD(%)	EBOD5(%)
240	2	0.3333	0.1583	67.34 69	63.8095
720	2	0.0694	0.0375	79.5918	74.2857
1200	2	0.0283	0.0083	86.1224	90.4761
240	4	0.5	0.375	51.0204	14.2857
720	4	0.1138	0.0902	66.5306	38.0952
1080	4	0.0546	0.05277	75.9183	45.71 42
240	6	0.75	0.4166	26.5306	4.7619
480	6	0.3	0.1833	41.2244	16.1904
960	6	0.0937	0.07812	63.2653	28.5714

Omil et al. studied the treatment of saline wastewater from a sea food –processing industry with the salinity content similar to sea water and they could be able to achieve 70-90% removal of organic matter.²⁵Rovirosa et al. inspected the treatment of saline wastewater with Down-Flow Anaerobic Fixed Bed Reactor (DFAFBR). They found that at the HRT of 24h and salt concentrations range of 5 g l^-1to 15 g l^-1, the reactor could reduce the COD concentration higher than 72%.²⁸Lefebvre等人进行了厌氧消化of tannery soak liquor characterized by high organic load and high salinity using Upflow Anaerobic Sludge Blanket reactor (UASB). They achieved a COD removal of 78% at a HRT of 5 days and a TDS concentration of 71gl_1.²⁹Kapdan and Erten studied the treatment of saline wastewater using up flow anaerobic packed bed reactor andHalanaerobium lacusrosei.They obtained a COD removal of 60% - 84% at the salt concentration of 3%.³⁰

Figure 4: The modified Stover-Kincannon model plotfor data showing removal of BOD5and COD byAspergillus oryzae. Click here to View figure

Figure 5: The modified Stover-Kincannon model plot fordata showing removal of BOD5and COD byHalobacillus dabanensis. Click here to View figure

The modified Stover-kincannon model is widely used for analyzing experimental data from continuously operated systems, especially in continuously operated anaerobic systems.^{31, 32}The shore model is a commonly used mathematical model applied to determine kinetic constant values of immobilized systems.³⁰The model is used in thermophilic treatment systems, such as continuous anaerobic filter treatment systems for treating paper-pulp liquors,³³anaerobic hybrid reactors³⁴and anaerobic filter for soybean wastewater treatment.³⁵According, the continuously operated aerobic systems were used in this study. As the plots in Fig 4 and Fig 5 show, the data of the 2 ml min^-1flow rate are in more adherences with this model than the other flow rates. Maximum removal of BOD₅and COD was obtained at a HRT of 1200 min, after theAspergillus oryzaeandHalobacillus dabanensisfilms were remained in the column for 43 h and 24 h respectively. The highest removal of BOD and COD byAspergillus oryzaewas occurred after 1200 min. Due to the rapid growth of theAspergillus oryzae, the biofilm was thickened, the column was reached the breakthrough point soon, and removal process was stopped. During the growth phase, the growth ofHalobacillus dabanensiswas stopped and interred to the death phase. Accordingly, the column reached to the breakthrough point.

According to the study based on the Stover model, the optimization conditions of the removal of COD efficiency constant of U_maxand K_Bwith the flow rate of 2 ml min^-1byAspergillus oryzaeandHalobacillus dabanensis, and the regression shown in Table 3.

Table 2: Effect of hydraulic retention time on COD and BOD₅loading rate and on efficiencies of COD and BOD₅
removal byHalobacillus dabanensis.

HRT (min)	Q (ml min-1)	VLRCOD (mg COD l-1min-1)	VLRBOD5 (mg BOD5l-1min-1)	ECOD(%)	EBOD5(%)
240	2	0.4166	0.275	59.1836	37.1428
720	2	0.0916	0.0430	73.4693	70.4761
1200	2	0.0375	0.0166	81.6326	80.9523
240	4	0.625	0.2875	38.7755	34.2857
720	4	0.1194	0.0597	64.8979	59.0476
1080	4	0.0648	0.025	71.4285	74.2857
240	6	0.8333	0.3166	18.3673	27.6190
480	6	0.3541	0.1291	30.6122	40.9523
960	6	0.1010	0.04479	60.4081	59.0476

Table 3: Comparison amount of U_maxand K_Band liner regression byAspergillus oryzaeandHalobacillus dabanensis

	Aspergillus oryzae	Halobacillus dabanensis
Umax(mg COD l-1min-1)	0.1449	0.1225
KB(mg COD l-1min-1)	0.0003	0.00082
R2(COD,Q 2ml min-1)	0.9523	0.9345
R2(BOD5问2毫升分钟-1)	0.8932	0.8642
R2(COD,Q 4ml min-1)	0.8661	0.8819
R2(BOD5, Q 4ml min-1)	0.8404	0.7818
R2(COD,Q 6ml min-1)	0.7952	0.8562
R2(BOD5, Q 6ml min-1)	0.8625	0.8761

Conclusions

In the present study, the removal of BOD₅and COD was investigated by a fixed bed operation, usingAspergillus oryzaeandHalobacillus dabanensis. The highest amounts of the BOD₅and COD removal were 90.4761% and 86.1224% byAspergillus oryzaewere achieved at the flow rate of 2 ml min^-1since. TheAspergillus oryzaefilm has a better performance than theHalobacillus dabanensisfilm. The formation ofAspergillus oryzaefilm around Ca-alginate boosts shelf-life of the biofilm on the substrate by polysaccharide compounds on the surface of fungi cells and hydrogen bonds between the polysaccharide compounds and Ca-alginate which causes maximum adhesion of the fungus on the bed. This causedAspergillus oryzaefilm to form regularly around Ca-alginate and provide maximum contact of the fungi with saline water. After 1200 minutes, the elimination process was stopped and the column reached the breakthrough point. The maximum substrate removal constant of 0.034 mg BOD₅l^-1min^-1and the saturation constant of 0.00026 mg BOD₅l^-1min^-1were calculated at the flow rate of 2 ml min^-1byHalobacillus dabanensis.

Acknowledgement

We express our deep gratitude to Mahmodieh Laboratory of Islamic Azad University- North Tehran Branch (IAU-NTB) for their final support. Special thanks for Genetic Engineering Central of Iran for their assistant in this project.

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