Most probable number (MPN) of bacteria shows the pollution load in water resources i.e. Shahpura Lake water. The widespread danger associcated with drinking water is bacterial contamination by sewage and other organic wastes. Coliforms are microbial indicator of drinking water pollution. Hence MPN count is investigated in the present study by mutiple tube technique, WHO (1979) recommended MPN 10/100 ml is fit for human consumption in the present study MPN count /100 ranged form 742/100 ml. Coliforms are recognized as microbial indicator of drinking water. Hence systematic monitoring and bacteriological analysis becomes important for public health and hygiene and is valuable for water management for water ecosystem and biodiversity.